› 4ltn

X-ray diffraction
2Å resolution

Crystal structures of NADH:FMN oxidoreductase (EMOB) - FMN, NADH complex

Released:
DOI: 10.2210/pdb4ltn/pdb
PDB entry 4ltn coloured by chain, front view.
PDB entry 4ltn coloured by chain, side view.
PDB entry 4ltn coloured by chain, top view.
Source organism: EDTA-degrading bacterium BNC1
Primary publication:
Crystal structures of NADH:FMN oxidoreductase (EmoB) at different stages of catalysis.
Nissen MS, Youn B, Knowles BD, Ballinger JW, Jun SY, Belchik SM, Xun L, Kang C
J Biol Chem 283 28710-20 (2008)
PMID: 18701448

Function and Biology Details

Reaction catalysed: 
FMNH(2) + NAD(+) = FMN + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent FMN reductase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 21.12 KDa
Source organism: EDTA-degrading bacterium BNC1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F9T2 (Residues: 1-197; Coverage: 100%)
Gene name: emoB
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand NAI 1 x NAI
2 bound ligands:
Ligand FMN 1 x FMN
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6422
Unit cell:
a: 101.18Å b: 101.18Å c: 129.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.191
Expression system: Escherichia coli

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Citations

1 review citation

Reducing alcohol levels in wines through rational and evolutionary engineering of Saccharomyces cerevisiae.
Tilloy et al. (2015)
20 other articles

2 mentions without citation

WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System.
Herrou et al. (2016)
1 more