› 4lto

X-ray diffraction
3.46Å resolution

Bacterial sodium channel in high calcium, I222 space group

Released:
DOI: 10.2210/pdb4lto/pdb
PDB entry 4lto coloured by chain, front view.
PDB entry 4lto coloured by chain, side view.
PDB entry 4lto coloured by chain, top view.
Source organism: Alkalilimnicola ehrlichii
Primary publication:
Structure of a prokaryotic sodium channel pore reveals essential gating elements and an outer ion binding site common to eukaryotic channels.
Shaya D, Findeisen F, Abderemane-Ali F, Arrigoni C, Wong S, Nurva SR, Loussouarn G, Minor DL
J Mol Biol 426 467-83 (2014)
PMID: 24120938

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.46 KDa
Source organism: Alkalilimnicola ehrlichii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0ABW0 (Residues: 143-288; Coverage: 51%)
Gene name: Mlg_0322
Sequence domains: Ion transport protein
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I222
Unit cell:
a: 147.669Å b: 161.679Å c: 162.234Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.268
Expression system: Escherichia coli

Quick links

Citations

22 review citations

The hitchhiker's guide to the voltage-gated sodium channel galaxy.
Ahern et al. (2016)
21 more

11 mentions without citation

Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart.
Payandeh et al. (2015)
10 more