› 4luq

X-ray diffraction
1.77Å resolution

Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3

Released:
DOI: 10.2210/pdb4luq/pdb
PDB entry 4luq coloured by chain, front view.
PDB entry 4luq coloured by chain, side view.
PDB entry 4luq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural Insights on the bacteriolytic and self-protection mechanism of muramidase effector Tse3 in Pseudomonas aeruginosa.
Li L, Zhang W, Liu Q, Gao Y, Gao Y, Wang Y, Wang DZ, Li Z, Wang T
J Biol Chem 288 30607-30613 (2013)
PMID: 24025333

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191146 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidoglycan muramidase Tse3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 46.61 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYC5 (Residues: 1-408; Coverage: 100%)
Gene names: PA3484, tse3
Immune protein Tsi3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 144 amino acids
Theoretical weight: 15.96 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYC4 (Residues: 23-145; Coverage: 95%)
Gene names: PA3485, tsi3
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002+, SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 63.333Å b: 81.288Å c: 111.414Å
α: 90° β: 93.64° γ: 90°
R-values:
R R work R free
0.139 0.139 0.188
Expression system: Escherichia coli

Quick links

Citations

5 review citations

VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors.
Durand et al. (2014)
4 more

2 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
1 more