› 4lvl

X-ray diffraction
2.2Å resolution

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt+3'Thiophosphate). Mn-bound crystal structure at pH 6.8

Released:
DOI: 10.2210/pdb4lvl/pdb
PDB entry 4lvl coloured by chain, front view.
PDB entry 4lvl coloured by chain, side view.
PDB entry 4lvl coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.
Pluta R, Boer DR, Lorenzo-Díaz F, Russi S, Gómez H, Fernández-López C, Pérez-Luque R, Orozco M, Espinosa M, Coll M
Proc Natl Acad Sci U S A 114 E6526-E6535 (2017)
PMID: 28739894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117807 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Plasmid recombination enzyme Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 23.17 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13925 (Residues: 2-199; Coverage: 40%)
Gene names: mob, pre
Sequence domains: Plasmid recombination enzyme
Structure domains: BirA Bifunctional Protein; domain 2
DNA (5'-D(*AP*CP*TP*TP*TP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*TP*AP*AP*AP*GP*TP*AP*TP*AP*GP*TP*GP*TP*GP*(TS6))-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.77 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6122
Unit cell:
a: 112.42Å b: 112.42Å c: 90.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Regulation of Gram-Positive Conjugation.
Kohler et al. (2019)
13 other articles

2 mentions without citation

The Facts and Family Secrets of Plasmids That Replicate via the Rolling-Circle Mechanism.
Garcillán-Barcia et al. (2022)
1 more