› 4lvm

X-ray diffraction
3.1Å resolution

MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (23nt). Mn-bound crystal structure at pH 6.5

Released:
DOI: 10.2210/pdb4lvm/pdb
PDB entry 4lvm coloured by chain, front view.
PDB entry 4lvm coloured by chain, side view.
PDB entry 4lvm coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.
Pluta R, Boer DR, Lorenzo-Díaz F, Russi S, Gómez H, Fernández-López C, Pérez-Luque R, Orozco M, Espinosa M, Coll M
Proc Natl Acad Sci U S A 114 E6526-E6535 (2017)
PMID: 28739894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117808 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Plasmid recombination enzyme Chains: A, C
Molecule details ›
Chains: A, C
Length: 198 amino acids
Theoretical weight: 23.17 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13925 (Residues: 2-199; Coverage: 40%)
Gene names: mob, pre
Sequence domains: Plasmid recombination enzyme
Structure domains: BirA Bifunctional Protein; domain 2
ACTTTAT oligonucleotide Chains: B, D
Molecule details ›
Chains: B, D
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: Streptococcus agalactiae
Expression system: Not provided
ATAAAGTATAGTGTGT oligonucleotide Chains: E, F
Molecule details ›
Chains: E, F
Length: 16 nucleotides
Theoretical weight: 4.98 KDa
Source organism: Streptococcus agalactiae
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand MG 1 x MG
Ligand CL 5 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 67.34Å b: 130.12Å c: 87.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.255 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Regulation of Gram-Positive Conjugation.
Kohler et al. (2019)
13 other articles

1 mention without citation

Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.
Pluta et al. (2017)