› 4lxg

X-ray diffraction
2.22Å resolution

Crystal structure of DxnB2, a carbon - carbon bond hydrolase from Sphingomonas wittichii RW1

Released:
DOI: 10.2210/pdb4lxg/pdb
PDB entry 4lxg coloured by chain, front view.
PDB entry 4lxg coloured by chain, side view.
PDB entry 4lxg coloured by chain, top view.
Source organism: Rhizorhabdus wittichii RW1
Primary publication:
The lid domain of the MCP hydrolase DxnB2 contributes to the reactivity toward recalcitrant PCB metabolites.
Ruzzini AC, Bhowmik S, Yam KC, Ghosh S, Bolin JT, Eltis LD
Biochemistry 52 5685-5695 (2013)
PMID: 23879719

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MCP Hydrolase Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.22 KDa
Source organism: Rhizorhabdus wittichii RW1
Expression system: Escherichia coli
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6522
Unit cell:
a: 66.983Å b: 66.983Å c: 327.743Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.234
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Shotgun proteomics suggests involvement of additional enzymes in dioxin degradation by Sphingomonas wittichii RW1.
Hartmann et al. (2014)
4 more

3 mentions without citation

Rerouting the Pathway for the Biosynthesis of the Side Ring System of Nosiheptide: The Roles of NosI, NosJ, and NosK.
Badding et al. (2017)
2 more