› 4lyd

X-ray diffraction
2.26Å resolution

Crystal structure of the S105A mutant of a C-C hydrolase, DxnB2 from Sphingomonas wittichii RW1

Released:
DOI: 10.2210/pdb4lyd/pdb
PDB entry 4lyd coloured by chain, front view.
PDB entry 4lyd coloured by chain, side view.
PDB entry 4lyd coloured by chain, top view.
Source organism: Rhizorhabdus wittichii RW1
Primary publication:
The lid domain of the MCP hydrolase DxnB2 contributes to the reactivity toward recalcitrant PCB metabolites.
Ruzzini AC, Bhowmik S, Yam KC, Ghosh S, Bolin JT, Eltis LD
Biochemistry 52 5685-5695 (2013)
PMID: 23879719

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MCP Hydrolase Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.21 KDa
Source organism: Rhizorhabdus wittichii RW1
Expression system: Escherichia coli DH5[alpha]
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6522
Unit cell:
a: 66.317Å b: 66.317Å c: 331.607Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.261
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

7 citation in other articles

Shotgun proteomics suggests involvement of additional enzymes in dioxin degradation by Sphingomonas wittichii RW1.
Hartmann et al. (2014)
6 more

2 mentions without citation

Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases.
Hajighasemi et al. (2016)
1 more