› 4lys

X-ray diffraction
1.83Å resolution

Crystal Structure of BRD4(1) bound to Colchiceine

Released:
DOI: 10.2210/pdb4lys/pdb
PDB entry 4lys coloured by chain, front view.
PDB entry 4lys coloured by chain, side view.
PDB entry 4lys coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
4-Acyl pyrroles: mimicking acetylated lysines in histone code reading.
Lucas X, Wohlwend D, Hügle M, Schmidtkunz K, Gerhardt S, Schüle R, Jung M, Einsle O, Günther S
Angew Chem Int Ed Engl 52 14055-9 (2013)
PMID: 24272870

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 15.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 44-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand 2SJ 1 x 2SJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 41.047Å b: 108.072Å c: 30.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Targeting bromodomains: epigenetic readers of lysine acetylation.
Filippakopoulos et al. (2014)
17 more

4 mentions without citation

Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli.
Lonardi et al. (2013)
3 more