› 4lzj

X-ray diffraction
2.4Å resolution

Crystal Structure of MurQ from H.influenzae with bound inhibitor

Released:
DOI: 10.2210/pdb4lzj/pdb
PDB entry 4lzj coloured by chain, front view.
PDB entry 4lzj coloured by chain, side view.
PDB entry 4lzj coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Structure of MurNAc 6-phosphate hydrolase (MurQ) from Haemophilus influenzae with a bound inhibitor.
Hadi T, Hazra S, Tanner ME, Blanchard JS
Biochemistry 52 9358-66 (2013)
PMID: 24251551

Function and Biology Details

Reaction catalysed: 
(R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-155217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylmuramic acid 6-phosphate etherase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 303 amino acids
Theoretical weight: 32.56 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44862 (Residues: 1-303; Coverage: 100%)
Gene names: HI_0754, murQ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 22H 2 x 22H
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Unit cell:
a: 76.492Å b: 113.335Å c: 143.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.225
Expression system: Escherichia coli

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Citations

1 review citation

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
2 other articles

1 mention without citation

Structure of MurNAc 6-phosphate hydrolase (MurQ) from Haemophilus influenzae with a bound inhibitor.
Hadi et al. (2013)