› 4m0g

X-ray diffraction
2.15Å resolution

The crystal structure of an adenylosuccinate synthetase from Bacillus anthracis str. Ames Ancestor.

Released:
DOI: 10.2210/pdb4m0g/pdb
PDB entry 4m0g coloured by chain, front view.
PDB entry 4m0g coloured by chain, side view.
PDB entry 4m0g coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Tan K, Zhou M, Zhang R, Kwon K, Anderson WF, Joachimiak A, Midwest Center for Structural Genomics (MCSG), Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 47.98 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81JI9 (Residues: 1-429; Coverage: 100%)
Gene names: BAS5320, BA_5716, GBAA_5716, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 76.498Å b: 76.498Å c: 342.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.243
Expression system: Escherichia coli BL21(DE3)

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