› 4m0v

X-ray diffraction
1.83Å resolution

Crystal structure of E.coli SbcD with Mn2+

Released:
DOI: 10.2210/pdb4m0v/pdb
PDB entry 4m0v coloured by chain, front view.
PDB entry 4m0v coloured by chain, side view.
PDB entry 4m0v coloured by chain, top view.
Source organism: Escherichia coli KO11FL
Primary publication:
Structural basis for DNA recognition and nuclease processing by the Mre11 homologue SbcD in double-strand breaks repair.
Liu S, Tian LF, Liu YP, An XM, Tang Q, Yan XX, Liang DC
Acta Crystallogr D Biol Crystallogr 70 299-309 (2014)
PMID: 24531464

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exonuclease subunit SbcD Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 354 amino acids
Theoretical weight: 39.47 KDa
Source organism: Escherichia coli KO11FL
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand MN 8 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P1
Unit cell:
a: 62.162Å b: 69.229Å c: 95.187Å
α: 72.24° β: 84° γ: 83.48°
R-values:
R R work R free
0.164 0.162 0.199
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Envisioning the dynamics and flexibility of Mre11-Rad50-Nbs1 complex to decipher its roles in DNA replication and repair.
Lafrance-Vanasse et al. (2015)
1 more

2 mentions without citation

Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme.
Fedoryshchak et al. (2020)
1 more