› 4m17

X-ray diffraction
2.1Å resolution

Crystal Structure of Surfactant Protein-D D325A/R343V mutant

Released:
DOI: 10.2210/pdb4m17/pdb
PDB entry 4m17 coloured by chain, front view.
PDB entry 4m17 coloured by chain, side view.
PDB entry 4m17 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanisms of inhibition of influenza by surfactant protein D revealed by large-scale molecular dynamics simulation.
Goh BC, Rynkiewicz MJ, Cafarella TR, White MR, Hartshorn KL, Allen K, Crouch EC, Calin O, Seeberger PH, Schulten K, Seaton BA
Biochemistry 52 8527-38 (2013)
PMID: 24224757

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pulmonary surfactant-associated protein D Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 147 amino acids
Theoretical weight: 15.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P35247 (Residues: 229-375; Coverage: 41%)
Gene names: COLEC7, PSPD, SFTP4, SFTPD
Sequence domains:
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

1 bound ligand:
Ligand CA 36 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 107.524Å b: 160.108Å c: 160.073Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.163 0.188
Expression system: Escherichia coli BL21

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Citations

12 review citations

Playing hide and seek: how glycosylation of the influenza virus hemagglutinin can modulate the immune response to infection.
Tate et al. (2014)
11 more

1 mention without citation

Molecular mechanisms of inhibition of influenza by surfactant protein D revealed by large-scale molecular dynamics simulation.
Goh et al. (2013)