› 4m1p

X-ray diffraction
2.56Å resolution

Crystal structure of the copper-sensing repressor CsoR with Cu(I) from Geobacillus thermodenitrificans NG80-2

Released:
DOI: 10.2210/pdb4m1p/pdb
PDB entry 4m1p coloured by chain, front view.
PDB entry 4m1p coloured by chain, side view.
PDB entry 4m1p coloured by chain, top view.
Source organism: Geobacillus thermodenitrificans NG80-2
Primary publication:
Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR).
Chang FM, Coyne HJ, Cubillas C, Vinuesa P, Fang X, Ma Z, Ma D, Helmann JD, García-de los Santos A, Wang YX, Dann CE, Giedroc DP
J Biol Chem 289 19204-17 (2014)
PMID: 24831014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-107347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-sensing transcriptional repressor CsoR Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.95 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4INJ9 (Residues: 2-105; Coverage: 99%)
Gene name: GTNG_1533
Sequence domains: Metal-sensitive transcriptional repressor
Structure domains: Metal-sensitive repressor, helix protomer

Ligands and Environments

2 bound ligands:
Ligand CU1 1 x CU1
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P622
Unit cell:
a: 89.204Å b: 89.204Å c: 57.985Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants.
Das et al. (2016)
4 more

6 mentions without citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
5 more