› 4m46

X-ray diffraction
2.7Å resolution

Crystal structure of a green-emitter native of Lampyris turkestanicus luciferase

Released:
DOI: 10.2210/pdb4m46/pdb
PDB entry 4m46 coloured by chain, front view.
PDB entry 4m46 coloured by chain, side view.
PDB entry 4m46 coloured by chain, top view.
Source organism: Lampyris turkestanicus
Primary publication:
Crystal structure of native and a mutant of Lampyris turkestanicus luciferase implicate in bioluminescence color shift.
Kheirabadi M, Sharafian Z, Naderi-Manesh H, Heineman U, Gohlke U, Hosseinkhani S
Biochim Biophys Acta 1834 2729-35 (2013)
PMID: 24103420

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 581 amino acids
Theoretical weight: 64.11 KDa
Source organism: Lampyris turkestanicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5UFR2 (Residues: 1-547; Coverage: 100%)
Sequence domains:
Structure domains: N-terminal domain of ligase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 85.1Å b: 85.1Å c: 96.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Split-luciferase complementary assay: applications, recent developments, and future perspectives.
Azad et al. (2014)
2 more

1 mention without citation

Beetle luciferases with naturally red- and blue-shifted emission.
Carrasco-López et al. (2018)