› 4m48

X-ray diffraction
2.96Å resolution

X-ray structure of dopamine transporter elucidates antidepressant mechanism

Released:
DOI: 10.2210/pdb4m48/pdb
PDB entry 4m48 coloured by chain, front view.
PDB entry 4m48 coloured by chain, side view.
PDB entry 4m48 coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structure of dopamine transporter elucidates antidepressant mechanism.
Penmatsa A, Wang KH, Gouaux E
Nature 503 85-90 (2013)
PMID: 24037379

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212460 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 60.94 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 21-601; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
9D5 antibody, light chain Chain: L
Molecule details ›
Chain: L
Length: 237 amino acids
Theoretical weight: 25.84 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
9D5 antibody, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 25.92 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand 21B 1 x 21B
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 97.839Å b: 133.684Å c: 162.443Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.256
Expression system: Homo sapiens

Quick links

Citations

73 review citations

SLC transporters as therapeutic targets: emerging opportunities.
Lin et al. (2015)
72 more

91 mentions without citation

Membrane transporters for the special amino acid glutamine: structure/function relationships and relevance to human health.
Pochini et al. (2014)
90 more