› 4m4l

X-ray diffraction
1.45Å resolution

The structure of Cu T6 bovine insulin

Released:
DOI: 10.2210/pdb4m4l/pdb
PDB entry 4m4l coloured by chain, front view.
PDB entry 4m4l coloured by chain, side view.
PDB entry 4m4l coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T(6) bovine insulin derivatives.
Frankaer CG, Mossin S, Ståhl K, Harris P
Acta Crystallogr D Biol Crystallogr 70 110-22 (2014)
PMID: 24419384

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134251 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.34 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P01317 (Residues: 85-105; Coverage: 26%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P01317 (Residues: 25-54; Coverage: 37%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: R3
Unit cell:
a: 81.36Å b: 81.36Å c: 33.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.169 0.204

Quick links

Citations

3 review citations

From protein structure to function via single crystal optical spectroscopy.
Ronda et al. (2015)
2 more

1 mention without citation

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T(6) bovine insulin derivatives.
Frankaer et al. (2014)