› 4m4o

X-ray diffraction
2Å resolution

Crystal structure of the aptamer minE-lysozyme complex

Released:
DOI: 10.2210/pdb4m4o/pdb
PDB entry 4m4o coloured by chain, front view.
PDB entry 4m4o coloured by chain, side view.
PDB entry 4m4o coloured by chain, top view.
Source organism: Gallus gallus
Entry authors: Malashkevich VN, Padlan FC, Toro R, Girvin M, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132869 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme
RNA (59-MER) Chain: B
Molecule details ›
Chain: B
Length: 59 nucleotides
Theoretical weight: 19 KDa
Source organism: Gallus gallus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 141.216Å b: 30.609Å c: 89.817Å
α: 90° β: 122.45° γ: 90°
R-values:
R R work R free
0.182 0.181 0.207
Expression systems:
  • Escherichia coli
  • Not provided

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