› 4m64

X-ray diffraction
3.35Å resolution

3D crystal structure of Na+/melibiose symporter of Salmonella typhimurium

Released:
DOI: 10.2210/pdb4m64/pdb
PDB entry 4m64 coloured by chain, front view.
PDB entry 4m64 coloured by chain, side view.
PDB entry 4m64 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structure-based mechanism for Na(+)/melibiose symport by MelB.
Ethayathulla AS, Yousef MS, Amin A, Leblanc G, Kaback HR, Guan L
Nat Commun 5 3009 (2014)
PMID: 24389923

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Melibiose permease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 486 amino acids
Theoretical weight: 54.24 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P30878 (Residues: 1-476; Coverage: 100%)
Gene names: STM4299, melB
Sequence domains: MFS/sugar transport protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P32
Unit cell:
a: 127.206Å b: 127.206Å c: 206.303Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.314 0.312 0.359
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
14 more

19 mentions without citation

Life at the border: adaptation of proteins to anisotropic membrane environment.
Pogozheva et al. (2014)
18 more