› 4m6f

X-ray diffraction
4.99Å resolution

Dimer of the G-Segment Invertase bound to a DNA substrate

Released:
DOI: 10.2210/pdb4m6f/pdb
PDB entry 4m6f coloured by chain, front view.
PDB entry 4m6f coloured by chain, side view.
PDB entry 4m6f coloured by chain, top view.
Source organisms:
Primary publication:
Exploiting large non-isomorphous differences for phase determination of a G-segment invertase-DNA complex.
Ritacco CJ, Steitz TA, Wang J
Acta Crystallogr D Biol Crystallogr 70 685-93 (2014)
PMID: 24598738

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117830 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Serine recombinase gin Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.71 KDa
Source organism: Escherichia virus Mu
UniProt:
  • Canonical: P03015 (Residues: 1-193; Coverage: 100%)
Gene names: Mup53, gin
Sequence domains:
gix site analog Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: synthetic construct
Expression system: Not provided
gix site analog Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E, NSLS BEAMLINE X29A
Spacegroup: P6422
Unit cell:
a: 119.8Å b: 119.8Å c: 343.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.229 0.288
Expression system: Not provided

Quick links

Citations

1 citations in other articles

Site-specific DNA Inversion by Serine Recombinases.
Johnson RC. (2015)

1 mention without citation

Exploiting large non-isomorphous differences for phase determination of a G-segment invertase-DNA complex.
Ritacco et al. (2014)