› 4m8j

X-ray diffraction
3.29Å resolution

Crystal structure of CaiT R262E bound to gamma-butyrobetaine

Released:
DOI: 10.2210/pdb4m8j/pdb
PDB entry 4m8j coloured by chain, front view.
PDB entry 4m8j coloured by chain, side view.
PDB entry 4m8j coloured by chain, top view.
Source organism: Proteus mirabilis ATCC 29906
Primary publication:
Arginine oscillation explains Na+ independence in the substrate/product antiporter CaiT.
Kalayil S, Schulze S, Kühlbrandt W
Proc Natl Acad Sci U S A 110 17296-301 (2013)
PMID: 24101465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-carnitine/gamma-butyrobetaine antiporter Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 56.29 KDa
Source organism: Proteus mirabilis ATCC 29906
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EY22 (Residues: 1-504; Coverage: 98%)
Gene names: PMI2654, caiT
Sequence domains: BCCT, betaine/carnitine/choline family transporter

Ligands and Environments

1 bound ligand:
Ligand NM2 1 x NM2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: R3
Unit cell:
a: 129.075Å b: 129.075Å c: 160.717Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.236 0.257
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A giant molecular proton pump: structure and mechanism of respiratory complex I.
Sazanov LA. (2015)
2 more

2 mentions without citation

Arginine oscillation explains Na+ independence in the substrate/product antiporter CaiT.
Kalayil et al. (2013)
1 more