Function and Biology Details
Reactions catalysed:
a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleosidein mRNA + S-adenosyl-L-homocysteine.
a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA +S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+).
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaacan be either Arg or Lys and Yaa can be either Ser or Ala.
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate+ phosphate + H(+).
ATP + H2O = ADP + phosphate + H(+).
Biochemical function:
Biological process:
- not assigned
Cellular component:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
RNA-directed RNA polymerase NS5 (preferred)
PDBe Complex ID:
PDB-CPX-146327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease NS3
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 26.33 KDa
Source organism: dengue virus type 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 247 amino acids
Theoretical weight: 26.33 KDa
Source organism: dengue virus type 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P12823 (Residues: 1394-1440, 1476-1660; Coverage: 7%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
NSLS BEAMLINE X6A
Spacegroup:
C2221
Expression system: Escherichia coli BL21(DE3)
