› 4ma6

X-ray diffraction
2Å resolution

Crystal structure of Ara h 8 with Epicatechin bound

Released:
DOI: 10.2210/pdb4ma6/pdb
PDB entry 4ma6 coloured by chain, front view.
PDB entry 4ma6 coloured by chain, side view.
PDB entry 4ma6 coloured by chain, top view.
Source organism: Arachis hypogaea
Primary publication:
Structure and function of the peanut panallergen Ara h 8.
Hurlburt BK, Offermann LR, McBride JK, Majorek KA, Maleki SJ, Chruszcz M
J Biol Chem 288 36890-901 (2013)
PMID: 24253038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bet v I/Major latex protein domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 16.97 KDa
Source organism: Arachis hypogaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VT83 (Residues: 1-157; Coverage: 100%)
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand 28E 1 x 28E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 40.647Å b: 88.957Å c: 42.657Å
α: 90° β: 96.58° γ: 90°
R-values:
R R work R free
0.195 0.192 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Food allergy: immune mechanisms, diagnosis and immunotherapy.
Yu et al. (2016)
8 more

7 mentions without citation

Allergens and their associated small molecule ligands-their dual role in sensitization.
Chruszcz et al. (2021)
6 more