› 4ma7

X-ray diffraction
1.97Å resolution

Crystal structure of mouse prion protein complexed with Promazine

Released:
DOI: 10.2210/pdb4ma7/pdb
PDB entry 4ma7 coloured by chain, front view.
PDB entry 4ma7 coloured by chain, side view.
PDB entry 4ma7 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of prion inhibition by phenothiazine compounds.
Baral PK, Swayampakula M, Rout MK, Kav NN, Spyracopoulos L, Aguzzi A, James MN
Structure 22 291-303 (2014)
PMID: 24373770

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209149 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major prion protein Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04925 (Residues: 116-229; Coverage: 49%)
Gene names: Prn-p, Prnp, Prp
Sequence domains: Prion/Doppel alpha-helical domain
POM1 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
POM1 light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand P2Z 1 x P2Z
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 83.211Å b: 106.038Å c: 75.733Å
α: 90° β: 95.68° γ: 90°
R-values:
R R work R free
0.217 0.216 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Approaches for discovering anti-prion compounds: lessons learned and challenges ahead.
Bolognesi et al. (2015)
8 more

7 mentions without citation

Transition of the prion protein from a structured cellular form (PrPC ) to the infectious scrapie agent (PrPSc ).
Baral et al. (2019)
6 more