› 4may

X-ray diffraction
2.2Å resolution

Crystal structure of an immune complex

Released:
DOI: 10.2210/pdb4may/pdb
PDB entry 4may coloured by chain, front view.
PDB entry 4may coloured by chain, side view.
PDB entry 4may coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crossreactivity of a human autoimmune TCR is dominated by a single TCR loop.
Sethi DK, Gordo S, Schubert DA, Wucherpfennig KW
Nat Commun 4 2623 (2013)
PMID: 24136005

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212218 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.76 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q30066 (Residues: 2-184; Coverage: 81%)
Gene name: HLA-DQA1
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 200 amino acids
Theoretical weight: 23.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q67AJ6 (Residues: 31-230; Coverage: 98%)
Gene name: HLA-DQB1
Sequence domains:
Structure domains:
HY.1B11 TCR alpha chain Chain: C
Molecule details ›
Chain: C
Length: 209 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
UL15 peptide-HY.1B11 TCR beta chain, chimeric construct Chain: D
Molecule details ›
Chain: D
Length: 266 amino acids
Theoretical weight: 29.39 KDa
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 72.514Å b: 123.176Å c: 134.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.236
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

6 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
5 more

8 mentions without citation

Human T cell receptor occurrence patterns encode immune history, genetic background, and receptor specificity.
DeWitt et al. (2018)
7 more