› 4mb8

X-ray diffraction
2.4Å resolution

Evolutionary history and metabolic insights of ancient mammalian uricases

Released:
DOI: 10.2210/pdb4mb8/pdb
PDB entry 4mb8 coloured by chain, front view.
PDB entry 4mb8 coloured by chain, side view.
PDB entry 4mb8 coloured by chain, top view.
Source organism: unclassified Mammalia
Primary publication:
Evolutionary history and metabolic insights of ancient mammalian uricases.
Kratzer JT, Lanaspa MA, Murphy MN, Cicerchi C, Graves CL, Tipton PA, Ortlund EA, Johnson RJ, Gaucher EA
Proc Natl Acad Sci U S A 111 3763-8 (2014)
PMID: 24550457

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 304 amino acids
Theoretical weight: 35.07 KDa
Source organism: unclassified Mammalia
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FZI9 (Residues: 1-304; Coverage: 100%)
Gene name: UOX
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 143.778Å b: 143.778Å c: 138.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.174 0.22
Expression system: Escherichia coli

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Citations

44 review citations

Hyperuricemia, Acute and Chronic Kidney Disease, Hypertension, and Cardiovascular Disease: Report of a Scientific Workshop Organized by the National Kidney Foundation.
Johnson et al. (2018)
43 more

5 mentions without citation

Evolutionary history and metabolic insights of ancient mammalian uricases.
Kratzer et al. (2014)
4 more