4mdx

X-ray diffraction
1.5Å resolution

Crystal structure of Bacillus subtilis MazF in complex with RNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-161233 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endoribonuclease EndoA Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.08 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96622 (Residues: 1-116; Coverage: 100%)
Gene names: BSU04660, mazF, ndoA, ydcE
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.
RNA, mRNA Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 66.793Å b: 69.885Å c: 79.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.178
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided