› 4mf6

X-ray diffraction
1.2Å resolution

Crystal structure of glutathione transferase BgramDRAFT_1843 from Burkholderia graminis, Target EFI-507289, with two glutathione molecules bound per one protein subunit

Released:
DOI: 10.2210/pdb4mf6/pdb
PDB entry 4mf6 coloured by chain, front view.
PDB entry 4mf6 coloured by chain, side view.
PDB entry 4mf6 coloured by chain, top view.
Source organism: Paraburkholderia graminis C4D1M
Entry authors: Patskovsky Y, Vetting MW, Toro R, Bhosle R, Al Obaidi N, Morisco LL, Wasserman SR, Sojitra S, Stead M, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Armstrong RN, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase domain Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.83 KDa
Source organism: Paraburkholderia graminis C4D1M
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1FXZ2 (Residues: 1-234; Coverage: 100%)
Gene names: BgramDRAFT_1843, R8871_03178, yghU
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GSH 2 x GSH
Ligand BEZ 1 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P43212
Unit cell:
a: 84.175Å b: 84.175Å c: 78.597Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.113 0.112 0.131
Expression system: Escherichia coli BL21(DE3)

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