› 4mgn

X-ray diffraction
3.2Å resolution

Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA

Released:
DOI: 10.2210/pdb4mgn/pdb
PDB entry 4mgn coloured by chain, front view.
PDB entry 4mgn coloured by chain, side view.
PDB entry 4mgn coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Primary publication:
Structural determinants for geometry and information decoding of tRNA by T box leader RNA.
Grigg JC, Ke A
Structure 21 2025-32 (2013)
PMID: 24095061

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195826 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
glyQS T box riboswitch Chains: A, C
Molecule details ›
Chains: A, C
Length: 86 nucleotides
Theoretical weight: 28.04 KDa
Source organism: Geobacillus kaustophilus
Expression system: Not provided
tRNA-glycine Chains: B, D
Molecule details ›
Chains: B, D
Length: 75 nucleotides
Theoretical weight: 24.33 KDa
Source organism: Geobacillus kaustophilus
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand MG 18 x MG
1 modified residue:
Ligand GTP 4 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 92.838Å b: 205.182Å c: 161.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.236 0.278
Expression system: Not provided

Quick links

Citations

8 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
7 more

14 mentions without citation

Structure and mechanism of the T-box riboswitches.
Zhang et al. (2015)
13 more