› 4mhg

X-ray diffraction
2.2Å resolution

Crystal structure of ETV6 bound to a specific DNA sequence

Released:
DOI: 10.2210/pdb4mhg/pdb
PDB entry 4mhg coloured by chain, front view.
PDB entry 4mhg coloured by chain, side view.
PDB entry 4mhg coloured by chain, top view.
Source organisms:
Primary publication:
Steric mechanism of auto-inhibitory regulation of specific and non-specific DNA binding by the ETS transcriptional repressor ETV6.
De S, Chan AC, Coyne HJ, Bhachech N, Hermsdorf U, Okon M, Murphy ME, Graves BJ, McIntosh LP
J Mol Biol 426 1390-406 (2014)
PMID: 24333486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117852 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor ETV6 Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 12.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P97360 (Residues: 329-426; Coverage: 20%)
Gene names: Etv6, Tel, Tel1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Specific 14 bp DNA Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: synthetic construct
Expression system: Not provided
Complementary Specific 14 bp DNA Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3121
Unit cell:
a: 57.582Å b: 57.582Å c: 130.481Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.221
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

ETV6 and ETV7: Siblings in hematopoiesis and its disruption in disease.
Rasighaemi et al. (2017)
2 more

11 mentions without citation

Germline ETV6 mutations in familial thrombocytopenia and hematologic malignancy.
Zhang et al. (2015)
10 more