› 4mht

X-ray diffraction
2.7Å resolution

TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH NATIVE DNA AND ADOHCY

Released:
DOI: 10.2210/pdb4mht/pdb
PDB entry 4mht coloured by chain, front view.
PDB entry 4mht coloured by chain, side view.
PDB entry 4mht coloured by chain, top view.
Source organism: Haemophilus haemolyticus
Primary publication:
Enzymatic C5-cytosine methylation of DNA: mechanistic implications of new crystal structures for HhaL methyltransferase-DNA-AdoHcy complexes.
O'Gara M, Klimasauskas S, Roberts RJ, Cheng X
J Mol Biol 261 634-45 (1996)
PMID: 8800212

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117853 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type II methyltransferase M.HhaI Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 37.04 KDa
Source organism: Haemophilus haemolyticus
UniProt:
  • Canonical: P05102 (Residues: 1-327; Coverage: 100%)
Gene name: hhaIM
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:
DNA (5'-D(*GP*AP*TP*AP*GP*(5CM)P*GP*CP*TP*AP*TP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Haemophilus haemolyticus
Expression system: Not provided
DNA (5'-D(*TP*GP*AP*TP*AP*GP*(5CM)P*GP*CP*TP*AP*TP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.98 KDa
Source organism: Haemophilus haemolyticus
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands
1 modified residue:
Ligand 5CM 2 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: R32
Unit cell:
a: 99.86Å b: 99.86Å c: 325.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Not provided

Quick links

Citations

13 review citations

Eukaryotic cytosine methyltransferases.
Goll et al. (2005)
12 more

7 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
6 more