› 4mi7

X-ray diffraction
1.65Å resolution

Crystal Structure of Salmonella Effector Protein GtgE

Released:
DOI: 10.2210/pdb4mi7/pdb
PDB entry 4mi7 coloured by chain, front view.
PDB entry 4mi7 coloured by chain, side view.
PDB entry 4mi7 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Primary publication:
Structural and enzymatic characterization of a host-specificity determinant from Salmonella.
Kohler AC, Spanò S, Galán JE, Stebbins CE
Acta Crystallogr D Biol Crystallogr 70 384-91 (2014)
PMID: 24531472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gifsy-2 prophage protein Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.95 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H9L447 (Residues: 79-214; Coverage: 60%)
Gene name: STM1055
Structure domains: Cathepsin B; Chain A

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 56.181Å b: 56.181Å c: 125.124Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Taking control: Hijacking of Rab GTPases by intracellular bacterial pathogens.
Spanò et al. (2018)
3 more

3 mentions without citation

The protease GtgE from Salmonella exclusively targets inactive Rab GTPases.
Wachtel et al. (2018)
2 more