› 4mif

X-ray diffraction
1.8Å resolution

Pyranose 2-oxidase from Phanerochaete chrysosporium, wild type from natural source

Released:
DOI: 10.2210/pdb4mif/pdb
PDB entry 4mif coloured by chain, front view.
PDB entry 4mif coloured by chain, side view.
PDB entry 4mif coloured by chain, top view.
Source organism: Phanerodontia chrysosporium
Primary publication:
Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.
Hassan N, Tan TC, Spadiut O, Pisanelli I, Fusco L, Haltrich D, Peterbauer CK, Divne C
FEBS Open Bio 3 496-504 (2013)
PMID: 24282677

Function and Biology Details

Reaction catalysed: 
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyranose 2-oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 620 amino acids
Theoretical weight: 69.32 KDa
Source organism: Phanerodontia chrysosporium
UniProt:
  • Canonical: Q6QWR1 (Residues: 1-620; Coverage: 100%)
Gene names: p2ox, pox
Sequence domains: GMC oxidoreductase

Ligands and Environments


Cofactor: Ligand FDA 4 x FDA
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P3221
Unit cell:
a: 164.034Å b: 164.034Å c: 232.521Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.153 0.171

Quick links

Citations

6 citation in other articles

Structural basis for binding of fluorinated glucose and galactose to Trametes multicolor pyranose 2-oxidase variants with improved galactose conversion.
Tan et al. (2014)
5 more

5 mentions without citation

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.
Bissaro et al. (2018)
4 more