› 4mj3

X-ray diffraction
1.7Å resolution

Haloalkane dehalogenase DmrA from Mycobacterium rhodesiae JS60

Released:
DOI: 10.2210/pdb4mj3/pdb
PDB entry 4mj3 coloured by chain, front view.
PDB entry 4mj3 coloured by chain, side view.
PDB entry 4mj3 coloured by chain, top view.
Source organism: Mycolicibacterium rhodesiae JS60
Primary publication:
Biochemical and biophysical characterisation of haloalkane dehalogenases DmrA and DmrB in Mycobacterium strain JS60 and their role in growth on haloalkanes.
Fung HK, Gadd MS, Drury TA, Cheung S, Guss JM, Coleman NV, Matthews JM
Mol Microbiol 97 439-53 (2015)
PMID: 25899475

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 325 amino acids
Theoretical weight: 35.78 KDa
Source organism: Mycolicibacterium rhodesiae JS60
Expression system: Escherichia coli
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand K 6 x K
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 84.22Å b: 79.079Å c: 95.761Å
α: 90° β: 92.52° γ: 90°
R-values:
R R work R free
0.166 0.165 0.19
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Impact of diesel and biodiesel contamination on soil microbial community activity and structure.
Mitter et al. (2021)
6 more

3 mentions without citation

Biochemical characterization of two haloalkane dehalogenases: DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea.
Carlucci et al. (2016)
2 more