› 4mj7

X-ray diffraction
2.51Å resolution

Crystal structure of the PIN domain of Saccharomyces cerevisiae Utp23

Released:
DOI: 10.2210/pdb4mj7/pdb
PDB entry 4mj7 coloured by chain, front view.
PDB entry 4mj7 coloured by chain, side view.
PDB entry 4mj7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural and functional analysis of Utp23, a yeast ribosome synthesis factor with degenerate PIN domain.
Lu J, Sun M, Ye K
RNA 19 1815-24 (2013)
PMID: 24152547

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171345 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA-processing protein UTP23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.21 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12339 (Residues: 1-159; Coverage: 63%)
Gene names: UTP23, YOR004W
Sequence domains: Fcf1
Structure domains: 5'-nuclease

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 91.427Å b: 91.427Å c: 92.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.288
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Comparison of preribosomal RNA processing pathways in yeast, plant and human cells - focus on coordinated action of endo- and exoribonucleases.
Tomecki et al. (2017)
1 more

4 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
3 more