› 4mkm

X-ray diffraction
1.75Å resolution

Repeat domains 1 & 2 of Clostridium perfringens Cpe0147

Released:
DOI: 10.2210/pdb4mkm/pdb
PDB entry 4mkm coloured by chain, front view.
PDB entry 4mkm coloured by chain, side view.
PDB entry 4mkm coloured by chain, top view.
Source organism: Clostridium perfringens B str. ATCC 3626
Primary publication:
Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin.
Kwon H, Squire CJ, Young PG, Baker EN
Proc Natl Acad Sci U S A 111 1367-72 (2014)
PMID: 24344302

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative surface anchored protein Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 38.65 KDa
Source organism: Clostridium perfringens B str. ATCC 3626
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1R775 (Residues: 292-625; Coverage: 17%)
Gene name: AC1_0147
Sequence domains: T-Q ester bond containing domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 48.188Å b: 63.448Å c: 104.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Engineering of Metabolic Pathways by Artificial Enzyme Channels.
Pröschel et al. (2015)
5 more

2 mentions without citation

Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin.
Kwon et al. (2014)
1 more