› 4mkn

X-ray diffraction
1.1Å resolution

Crystal structure of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii at 1.1 A of resolution

Released:
DOI: 10.2210/pdb4mkn/pdb
Model geometry
Fit model/data
PDB entry 4mkn coloured by chain, front view.
PDB entry 4mkn coloured by chain, side view.
PDB entry 4mkn coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
High-resolution crystal structure and redox properties of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii.
Zaffagnini M, Michelet L, Sciabolini C, Di Giacinto N, Morisse S, Marchand CH, Trost P, Fermani S, Lemaire SD
Mol Plant 7 101-20 (2014)
PMID: 24157611

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloroplast triosephosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 28.8 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S7Y5 (Residues: 13-282; Coverage: 96%)
Gene names: CHLRE_01g029300v5, TIM
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand MRD 5 x MRD
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 59.814Å b: 93.211Å c: 100.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.177
Expression system: Escherichia coli

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Citations

4 review citations

Redox regulation of the Calvin-Benson cycle: something old, something new.
Michelet et al. (2013)
3 more