› 4mkp

X-ray diffraction
1.95Å resolution

Crystal structure of human cGAS apo form

Released:
DOI: 10.2210/pdb4mkp/pdb
PDB entry 4mkp coloured by chain, front view.
PDB entry 4mkp coloured by chain, side view.
PDB entry 4mkp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analyses of DNA-sensing and immune activation by human cGAS.
Kato K, Ishii R, Goto E, Ishitani R, Tokunaga F, Nureki O
PLoS One 8 e76983 (2013)
PMID: 24116191

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 42.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 161-522; Coverage: 69%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 123.546Å b: 48.311Å c: 59.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.252
Expression system: Escherichia coli

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Citations

14 review citations

Discriminating self from non-self in nucleic acid sensing.
Schlee et al. (2016)
13 more

4 mentions without citation

Molecular and Structural Basis of DNA Sensors in Antiviral Innate Immunity.
Zahid et al. (2020)
3 more