› 4mks

X-ray diffraction
2.08Å resolution

Crystal structure of enolase from Lactobacillus gasseri

Released:
DOI: 10.2210/pdb4mks/pdb
PDB entry 4mks coloured by chain, front view.
PDB entry 4mks coloured by chain, side view.
PDB entry 4mks coloured by chain, top view.
Source organism: Lactobacillus gasseri ATCC 33323 = JCM 1131
Primary publication:
Crystal structure of an efficacious gonococcal adherence inhibitor: an enolase from Lactobacillus gasseri.
Raghunathan K, Harris PT, Spurbeck RR, Arvidson CG, Arvidson DN
FEBS Lett 588 2212-6 (2014)
PMID: 24859038

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-170016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 48 KDa
Source organism: Lactobacillus gasseri ATCC 33323 = JCM 1131
Expression system: Escherichia coli
UniProt:
  • Canonical: Q042F4 (Residues: 1-432; Coverage: 100%)
Gene names: LGAS_1305, eno2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I4
Unit cell:
a: 145.24Å b: 145.24Å c: 99.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.234
Expression system: Escherichia coli

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Citations

5 citation in other articles

A Moonlighting Enolase from Lactobacillus gasseri does not Require Enzymatic Activity to Inhibit Neisseria gonorrhoeae Adherence to Epithelial Cells.
Spurbeck et al. (2015)
4 more

1 mention without citation

Molecular Docking of Bacterial Protein Modulators and Pharmacotherapeutics of Carica papaya Leaves as a Promising Therapy for Sepsis: Synchronising In Silico and In Vitro Studies.
Usmani et al. (2023)