› 4mlb

X-ray diffraction
2.35Å resolution

Reverse polarity of binding pocket suggests different function of a MOP superfamily transporter from Pyrococcus furiosus Vc1 (DSM3638)

Released:
DOI: 10.2210/pdb4mlb/pdb
PDB entry 4mlb coloured by chain, front view.
PDB entry 4mlb coloured by chain, side view.
PDB entry 4mlb coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Inward-facing conformation of a multidrug resistance MATE family transporter.
Zakrzewska S, Mehdipour AR, Malviya VN, Nonaka T, Koepke J, Muenke C, Hausner W, Hummer G, Safarian S, Michel H
Proc Natl Acad Sci U S A 116 12275-12284 (2019)
PMID: 31160466

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-186449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MATE family efflux transporter Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 492 amino acids
Theoretical weight: 52.93 KDa
Source organism: Pyrococcus furiosus
UniProt:
  • Canonical: Q8U2X0 (Residues: 1-461; Coverage: 100%)
Gene name: PF0708
Sequence domains: MatE

Ligands and Environments

2 bound ligands:
Ligand CXE 53 x CXE
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: C2
Unit cell:
a: 219.56Å b: 94.548Å c: 138.822Å
α: 90° β: 126.47° γ: 90°
R-values:
R R work R free
0.204 0.203 0.231

Quick links

Citations

5 review citations

The Bacterial Cell Wall: From Lipid II Flipping to Polymerization.
Kumar et al. (2022)
4 more

6 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
5 more