› 4mlo

X-ray diffraction
1.65Å resolution

1.65A resolution structure of ToxT from Vibrio cholerae (P21 Form)

Released:
DOI: 10.2210/pdb4mlo/pdb
PDB entry 4mlo coloured by chain, front view.
PDB entry 4mlo coloured by chain, side view.
PDB entry 4mlo coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
1.65 Å resolution structure of the AraC-family transcriptional activator ToxT from Vibrio cholerae.
Li J, Wehmeyer G, Lovell S, Battaile KP, Egan SM
Acta Crystallogr F Struct Biol Commun 72 726-31 (2016)
PMID: 27599865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH araC/xylS-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BGC0 (Residues: 1-276; Coverage: 100%)
Gene names: D6U24_13630, ERS013200_01425, tcpN, toxT
Sequence domains: Helix-turn-helix domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand PAM 1 x PAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 47.337Å b: 39.41Å c: 80.238Å
α: 90° β: 97.94° γ: 90°
R-values:
R R work R free
0.169 0.168 0.194
Expression system: Escherichia coli

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Citations

2 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more

2 mentions without citation

1.65 Å resolution structure of the AraC-family transcriptional activator ToxT from Vibrio cholerae.
Li et al. (2016)
1 more