› 4mnd

X-ray diffraction
2.66Å resolution

Crystal structure of Archaeoglobus fulgidus IPCT-DIPPS bifunctional membrane protein

Released:
DOI: 10.2210/pdb4mnd/pdb
PDB entry 4mnd coloured by chain, front view.
PDB entry 4mnd coloured by chain, side view.
PDB entry 4mnd coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism.
Nogly P, Gushchin I, Remeeva A, Esteves AM, Borges N, Ma P, Ishchenko A, Grudinin S, Round E, Moraes I, Borshchevskiy V, Santos H, Gordeliy V, Archer M
Nat Commun 5 4169 (2014)
PMID: 24942835

Function and Biology Details

Reactions catalysed: 
CTP + 1L-myo-inositol 1-phosphate = diphosphate + CDP-1L-myo-inositol
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate = CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional IPC transferase and DIPP synthase Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 53.63 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29976 (Residues: 55-490; Coverage: 89%)
Gene name: AF_0263
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LFA 4 x LFA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 41.369Å b: 107.584Å c: 123.953Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.3
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Bacterial membrane lipids: diversity in structures and pathways.
Sohlenkamp et al. (2016)
7 more

5 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
4 more