› 4mpf

X-ray diffraction
2.1Å resolution

Crystal structure of human glutathione transferase theta-2, complex with inorganic phosphate, GSH free, target EFI-507257

Released:
DOI: 10.2210/pdb4mpf/pdb
PDB entry 4mpf coloured by chain, front view.
PDB entry 4mpf coloured by chain, side view.
PDB entry 4mpf coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Imker HJ, Al Obaidi N, Stead M, Love J, Gerlt JA, Armstrong RN, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase theta-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 266 amino acids
Theoretical weight: 30.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG29 (Residues: 1-244; Coverage: 100%)
Gene name: GSTT2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TLA 1 x TLA
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 92.934Å b: 92.934Å c: 119.022Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.235
Expression system: Escherichia coli BL21(DE3)

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