› 4mrp

X-ray diffraction
2.5Å resolution

Structure of a bacterial Atm1-family ABC transporter

Released:
DOI: 10.2210/pdb4mrp/pdb
PDB entry 4mrp coloured by chain, front view.
PDB entry 4mrp coloured by chain, side view.
PDB entry 4mrp coloured by chain, top view.
Source organism: Novosphingobium aromaticivorans DSM 12444
Primary publication:
Structural basis for heavy metal detoxification by an Atm1-type ABC exporter.
Lee JY, Yang JG, Zhitnitsky D, Lewinson O, Rees DC
Science 343 1133-6 (2014)
PMID: 24604198

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATM1-type heavy metal exporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 68.38 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2G506 (Residues: 1-608; Coverage: 100%)
Gene names: Saro_2631, atm1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand LDA 4 x LDA
Ligand GSH 2 x GSH
Ligand PO4 4 x PO4
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 321.853Å b: 95.262Å c: 79.01Å
α: 90° β: 102.01° γ: 90°
R-values:
R R work R free
0.204 0.202 0.231
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
21 more

6 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
5 more