› 4mso

X-ray diffraction
1.4Å resolution

X-ray crystal structure of a serine hydroxymethyl transferase in apo form from Burkholderia cenocepacia

Released:
DOI: 10.2210/pdb4mso/pdb
PDB entry 4mso coloured by chain, front view.
PDB entry 4mso coloured by chain, side view.
PDB entry 4mso coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 45.98 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4ECY9 (Residues: 1-415; Coverage: 100%)
Gene names: BCAL3197, glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 55.28Å b: 72.58Å c: 182.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.182
Expression system: Escherichia coli

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