› 4mst

X-ray diffraction
1.93Å resolution

Crystal Structure of a putative catalytic domain of a chitinase-like protein (HbCLP1) from Hevea brasiliensis

Released:
DOI: 10.2210/pdb4mst/pdb
PDB entry 4mst coloured by chain, front view.
PDB entry 4mst coloured by chain, side view.
PDB entry 4mst coloured by chain, top view.
Source organism: Hevea brasiliensis
Primary publication:
Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
Martínez-Caballero S, Cano-Sánchez P, Mares-Mejía I, Díaz-Sánchez AG, Macías-Rubalcava ML, Hermoso JA, Rodríguez-Romero A
FEBS J 281 4535-54 (2014)
PMID: 25104038

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inactive chitinase-like protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 26.55 KDa
Source organism: Hevea brasiliensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q949H3 (Residues: 73-314; Coverage: 82%)
Gene names: CHI-L1, RQ30
Sequence domains: Chitinase class I
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Unit cell:
a: 111.106Å b: 40.415Å c: 111.854Å
α: 90° β: 96.73° γ: 90°
R-values:
R R work R free
0.168 0.166 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Chitin and chitinase: Role in pathogenicity, allergenicity and health.
Patel et al. (2017)
2 more

1 mention without citation

Genomic analysis and chitinase characterization of Vibrio harveyi WXL538: insight into its adaptation to the marine environment.
Ran et al. (2023)