› 4mx3

X-ray diffraction
3.88Å resolution

Crystal Structure of PKA RIalpha Homodimer

Released:
DOI: 10.2210/pdb4mx3/pdb
PDB entry 4mx3 coloured by chain, front view.
PDB entry 4mx3 coloured by chain, side view.
PDB entry 4mx3 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
PKA RIα homodimer structure reveals an intermolecular interface with implications for cooperative cAMP binding and Carney complex disease.
Bruystens JG, Wu J, Fortezzo A, Kornev AP, Blumenthal DK, Taylor SS
Structure 22 59-69 (2014)
PMID: 24316401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type I-alpha regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 42.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00514 (Residues: 2-380; Coverage: 100%)
Gene name: PRKAR1A
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CMP 4 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 104.672Å b: 104.672Å c: 218.327Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.26 0.287
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From structure to the dynamic regulation of a molecular switch: A journey over 3 decades.
Taylor et al. (2021)
16 other articles

8 mentions without citation

Allostery, and how to define and measure signal transduction.
Nussinov et al. (2022)
7 more