› 4mxk

X-ray diffraction
1.52Å resolution

X-ray structure of Fe(II)-ZnPIXFeBMb1

Released:
DOI: 10.2210/pdb4mxk/pdb
PDB entry 4mxk coloured by chain, front view.
PDB entry 4mxk coloured by chain, side view.
PDB entry 4mxk coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Spectroscopic and computational study of a nonheme iron nitrosyl center in a biosynthetic model of nitric oxide reductase.
Chakraborty S, Reed J, Ross M, Nilges MJ, Petrik ID, Ghosh S, Hammes-Schiffer S, Sage JT, Zhang Y, Schulz CE, Lu Y
Angew Chem Int Ed Engl 53 2417-21 (2014)
PMID: 24481708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.28 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand ZNH 1 x ZNH
Ligand FE2 2 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 39.75Å b: 47.593Å c: 76.008Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.22
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metal-binding promiscuity in artificial metalloenzyme design.
Pordea A. (2015)
1 more

2 mentions without citation

Spectroscopic and computational study of a nonheme iron nitrosyl center in a biosynthetic model of nitric oxide reductase.
Chakraborty et al. (2014)
1 more