› 4mxp

X-ray diffraction
1.83Å resolution

Structural Basis for PI(4)P-Specific Membrane Recruitment of the Legionella pneumophila Effector DrrA/SidM

Released:
DOI: 10.2210/pdb4mxp/pdb
PDB entry 4mxp coloured by chain, front view.
PDB entry 4mxp coloured by chain, side view.
PDB entry 4mxp coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Structural basis for PI(4)P-specific membrane recruitment of the Legionella pneumophila effector DrrA/SidM.
Del Campo CM, Mishra AK, Wang YH, Roy CR, Janmey PA, Lambright DG
Structure 22 397-408 (2014)
PMID: 24530282

Function and Biology Details

Reaction catalysed: 
GTP + [protein]-L-tyrosine = diphosphate + [protein]-O(4)-(5'-guanylyl)-L-tyrosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multifunctional virulence effector protein DrrA Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.11 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZSQ3 (Residues: 330-647; Coverage: 49%)
Gene names: drrA, lpg2464, sidM
Sequence domains:
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand DB4 1 x DB4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: I41
Unit cell:
a: 128.552Å b: 128.552Å c: 53.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.208
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Formation of a pathogen vacuole according to Legionella pneumophila: how to kill one bird with many stones.
Finsel et al. (2015)
6 more

5 mentions without citation

Polyphosphoinositide-Binding Domains: Insights from Peripheral Membrane and Lipid-Transfer Proteins.
Pemberton et al. (2019)
4 more