› 4mxr

X-ray diffraction
1.85Å resolution

Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2

Released:
DOI: 10.2210/pdb4mxr/pdb
PDB entry 4mxr coloured by chain, front view.
PDB entry 4mxr coloured by chain, side view.
PDB entry 4mxr coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme.
Hai Y, Dugery RJ, Healy D, Christianson DW
Biochemistry 52 9294-309 (2013)
PMID: 24261485

Function and Biology Details

Reaction catalysed: 
N-formimidoyl-L-glutamate + H(2)O = L-glutamate + formamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 34.64 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4DSA0 (Residues: 1-308; Coverage: 100%)
Gene name: Tc00.1047053507031.90
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 128.922Å b: 128.922Å c: 85.377Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.217
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Trypanosomatid Infections: How Do Parasites and Their Excreted-Secreted Factors Modulate the Inducible Metabolism of l-Arginine in Macrophages?
Holzmuller et al. (2018)
3 other articles

1 mention without citation

Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme.
Hai et al. (2013)