› 4my2

X-ray diffraction
2.4Å resolution

Crystal Structure of Norrin in fusion with Maltose Binding Protein

Released:
DOI: 10.2210/pdb4my2/pdb
PDB entry 4my2 coloured by chain, front view.
PDB entry 4my2 coloured by chain, side view.
PDB entry 4my2 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and function of Norrin in assembly and activation of a Frizzled 4-Lrp5/6 complex.
Ke J, Harikumar KG, Erice C, Chen C, Gu X, Wang L, Parker N, Cheng Z, Xu W, Williams BO, Melcher K, Miller LJ, Xu HE
Genes Dev 27 2305-19 (2013)
PMID: 24186977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Norrin Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 52.84 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
  • Canonical: Q00604 (Residues: 30-133; Coverage: 95%)
Gene names: EVR2, JW3994, NDP, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 59.458Å b: 79.022Å c: 104.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.237
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
20 more

5 mentions without citation

The DAN family: modulators of TGF-β signaling and beyond.
Nolan et al. (2014)
4 more